Solubilization of substance P binding sites from rat brain membranes.
نویسندگان
چکیده
منابع مشابه
Solubilization and characterization of substance P-binding sites from chick brain membranes.
Sites binding monoiodinated-Bolton-Hunter-reagent-labelled substance P were solubilized from 1-day-old-chick brain membrane by using non-ionic detergents (1% digitonin/1% n-octyl glucoside) and a high concentration of NaCl (0.5 M). The solubilized preparation retained the pharmacological properties of the high-affinity binding sites found in the native membrane. The high density of specific bin...
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The ontogeny of substance P (SP) receptor binding sites in rat brain has been studied using both membrane binding assays and in vitro receptor autoradiography. The density of SP binding sites is maximal 1 d before birth and decreases thereafter to reach adult values 14 d after birth. During the early postnatal period, the distribution of SP binding sites undergoes major modifications. For examp...
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Purification and characterization of Na+ channel protein from mammalian ventricular myocytes has heretofore been complicated by the low concentration of Na+ channels and by the finding that mammalian ventricles contain both tetrodotoxin (TTX)-sensitive channels (TSC), with high affinity for saxitoxin (STX), and TTX-insensitive channels (TIC), with low affinity for STX. Most (perhaps all) of the...
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High-affinity [3H]serotonin binding activity has been solubilized from bovine cerebral cortical membranes by using Triton X-100, Tween-80, and octyl-beta-D-glucopyranoside. This mixture of detergents solubilizes the high-affinity [3H]serotonin binding activity present in crude membrane preparations with retention of 75-90% specific binding. The detergent mixture was chosen because it can easily...
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Rat brain cortex membranes bind to a conjugate of substance P and 12SI-labeled Bolton-Hunter reagent, and this binding can be inhibited by a low concentration of substance P ( K d = 1.2 f 0.4 X lo-* M). This binding is reversible and saturable (0.5 f 0.1 pmol of binding sites/mg of protein). Fragments of substance P as small as the carboxyl-terminal hexapeptide can inhibit the binding although ...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1985
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)63662-0